How to Mix HCG (Human Gonadotropin)

Step 1: Wash your hands and gather your materials.

First, you want to thoroughly wash your hands with soap and water for at least 20 seconds.

After that, collect and prep your materials. You’ll need an alcohol wipe, your bacteriostatic water and HCG vials, your reconstitution syringe and needle, and a Sharps container for safe disposal.

If you are planning to inject after reconstituting your HCG, you can also prep a second syringe, needle, and alcohol wipe. (Avoid using the same needle to limit contamination and to avoid a dull needle point).

Step 2: Prep your materials.

Remove the caps from your bacteriostatic water and HCG vial, and use an alcohol wipe to sterilize the top of each vial.

Add the needle to your syringe, careful not to touch the needle with your fingers. Do not let the needle rest on any other surface or come into contact with anything – this can lead to contamination.

If you are planning to inject afterwards, place your second needle on its syringe (avoid touching the tip of the needle while doing so). Also make sure your second alcohol wipe is easily within reach, so you can clean the skin of the injection site prior to injecting.

Step 3: Draw up your bacteriostatic water.  

The amount of bacteriostatic water you add depends on the size of your vial. HCG typically comes in a 10,000iu vial, which means you’ll need 10 mL ML/CC (milliliters per cubic centimeter) of bacteriostatic water.

The measurements on your syringe should be in milliliters (ML). Pull air into the syringe up to the required amount (which is typically 10 mL, but check your pharmacy information to be sure).

Hold the bacteriostatic water upside down and pierce the stopper with your needle.

Slowly inject air into the vial. If you feel any pressure, stop, and allow the syringe to adjust. Then begin pressing the plunger again until all the air is out of the vial, and the bacteriostatic water has entered the syringe to the appropriate amount.

As always, avoid letting the needle come into contact with anything – this way you avoid contaminating your HCG vial.

Step 4: Inject bacteriostatic water into lyophilized HGC.

Pierce the stopper of the HCG vial with the needle of your water-filled syringe.

Slowly push the bacteriostatic water into the vial. If you feel pressure, stop, and let it equalize before moving forward. The plunger may push back upwards several times before you are finished.

Do not force the water into the HCG vial; go slowly and carefully.

Step 5: Mix your HCG.

Gently swirl the solution until the powder and the bacteriostatic water have fully combined. Do not shake your medication or aggressively mix it. Once you’re finished, you should no longer see any powder in the HCG vial.

Step 6: Inject your HCG if desired.

If you’re ready to inject your HCG, utilize your second needle, syringe, and alcohol wipe now.

See more detailed instructions for administering subcutaneous injections. HCG injections are typically done subcutaneously, but make sure to follow the guidance of your medical provider and pharmacy.

Step 7: Store your HCG.

After it’s reconstituted, immediately refrigerate your HCG.

Prior to mixing, powdered HCG can be kept at room temperature. But once you add the bacteriostatic water, it should be kept in the fridge.

Reconstituted HCG can typically be used for up to 60-90 days from the date of reconstitution.

If you accidentally leave your HCG out of the fridge, the medication is likely still fine to use, but avoid leaving it out for 72 hours or more.